ExFHPB-1 Determination of Extraneous Material in Alimentary Paste Products
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ED46949BE4D347B290760609B8594B45 |
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文件大小(MB)： |
0.03 |
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页数： |
7 |
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日期： |
2012-3-3 |
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Published on the Food Directorate’s (Health Canada's) website at,http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/index_e.php,Government of Canada Gouvernement du Canada,HPB Method ExFHPB-1,March 2009,HEALTH PRODUCTS AND FOOD BRANCH,OTTAWA,DETERMINATION OF EXTRANEOUS MATERIAL IN ALIMENTARY PASTE PRODUCTS,Eva Pietrzak,National Manager,Food Microbiology and Extraneous Matter Evaluation Section,Food Safety Division, CFIA,Tower 2, 4th floor, Rm 153,1440 Merivale,Ottawa, ON.,K1A 0Y9,E-mail: Eva.Pietrzak@inspection.gc.ca,1. APPLICATION,This method is applicable to the enumeration of extraneous material light filth elements, such as insects, insect,fragments, mites, hairs, and feather barbules, and heavy filth particles, such as sand, soil, metal, glass, insect,and rodent excreta pellets, in alimentary paste products to determine compliance with Sections 4, 5 and 7 of,the Food and Drugs Act. This method replaces ExFHPB-1 dated January 2006.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or shipped,under conditions as nearly uniform as possible, preferably designated by a common container code or marking,and in any event, consisting of not more than one variety, grade or type of product from a single identifiable,source.,3. COLLECTION OF SAMPLES,3.1 Scrutinize the entire lot for live infestation. If live infestation is found, do not sample until after,fumigation or other effective treatment.,3.2 Obtain three sample units selected at random from the lot of at least 225 g each using appropriate,sampling equipment and containers. Three sample units constitute a sample.,3.3 Each sample unit must be kept separate and labelled 1, 2, 3. Complete information respecting the,lot size, weight of individual containers, country of origin, exporter, importer or domestic manufacturer,and product and lot identification should be recorded and should accompany the sample.,4. MATERIALS AND SPECIAL EQUIPMENT,1) Balance,2) 2 - 2.0 L beakers and lids (watch glasses),ExFHPB-01,- 2 - March 2009,3) Autoclave. Either (a) slow exhaust type (Set slow exhaust to lower pressure from 1.15 to 0 kg per sq.,cm in 15-20 min.) or (b) non-slow exhaust (Let cool to 0 kg per sq. cm before opening or venting),4) U.S. standard No. 230 sieve,5) Shallow pan $ 20 cm in diameter,6) Smooth magnetic stirring bar, teflon coated (1 x 5 cm),7) Stirrer hot plate,8) 2 L percolator, premarked at 250 and 1700 mL levels,9) Wash bottles,10) Rubber policeman,11) Suction filtration apparatus with Buchner or Hirsch funnel (5-7 cm perforated plate),12) Ruled filter paper. Filter paper should be larger than funnel plate (7-9 cm),13) Petri dishes to suit size of filter paper used,14) Stereoscopic microscope (10-30x),15) Ashless filter paper. Filter paper should be larger than funnel plate (7-9 cm),16) Drying oven (40-60oC),17) Crucible,18) Desiccator,19) Muffle oven,20) Concentrated HCl,21) Antifoam solution. BDH Antifoam B,22) Detergent, 1% sodium lauryl sulphate,23) Mineral oil. Paraffin oil, white, light 125/135 Saybolt Universal Viscosity (38oC), specific gravity 0.840-,0.860 (24oC),24) Alcohol, either 95% ethanol or 99% isopropanol,25) Glycerol-alcohol (95%) mixture (1:1),26) Chloroform (CHCl3),5. PROCEDURE,The examination shall be carried out in accordance with the following instructions.,5.1 Preparation of Analytical Units,5.1.1 Thoroughly mix a sample unit and weigh 225 g of alimentary paste. This 225 g constitutes an,analytical unit.,ExFHPB-01,- 3 - March 2009,5.1.2 Repeat step 5.1.1 for remaining two sample units.,5.2 Isolation - Light Filth,5.2.1 Transfer an analytical unit to a 2 L beaker. For spaghetti, break into lengths that will not lie,flat on bottom of beaker. If large amounts of spaghetti lie on the bottom they may not digest,completely.,5.2.2 Add 1 L HCl (30 mL concentrated HCl + 970 mL water), 0.3 mL of antifoam solution and,cover with a lid. Set beaker in a larger container in case of breakage.,5.2.3 Autoclave for 30 min. at 121oC. Caution: check autoclave periodically for corrosive damage.,5.2.4 Wet sieve on a No. 230 sieve with hot tap water (50-70oC) to remove all original liquid and,a major portion of fine material.,5.2.5 If there is any fat material remaining defat as follows. Place sieve in a shallow pan, cover,residue to a depth of approximately 2 cm with hot (50-70oC) detergent, leave for 5 min., drain,and wet sieve with hot tap water until all suds have washed through. Be careful not to allow,suds to foam over sides of sieve. Repeat defatting procedure at least once or until all visible,fat has been removed.,5.2.6 Transfer sieve re……
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